Optimization of Bio-parameters of Fermentation Medium for L-Glutaminase Production by using Aspergillus flavus Strain S4

Abstract

Due to its potential for treating lymphoblastic leukemia, the amidohydrolase enzyme L-glutaminase has attracted a lot of attention. Using a variety of agro-industrial residues, this study aims to maximize the extracellular synthesis of L-glutaminase by Aspergillus flavus strain S4. To identify ideal production conditions, the effects of important process variables were assessed, such as substrate type, incubation time, temperature, moisture content, starting pH, additional carbon and nitrogen sources, and metal ions. On the second day of incubation, at pH 6.0, 30°C, and 50% moisture content, A. flavus S4 was cultivated on tea dust and produced the most glutaminase under ideal conditions using solid-state fermentation (SSF). The addition of sodium nitrite and dextrose as dietary elements improved production even further.